Application of computational tools and chemical engineering principles for a molecular understanding of human cellular processes from biological big data (transcriptomics, proteomics, PTMs, and metabolomics).
Main current projects are investigating the following topics:
PRIN 2022: “Identification of new disease specific omic signatures and therapeutic targets for Glycogen storage disease type Ia” (CUP J53D23009000006)
Molecular mechanisms of nuclear envelope deformation. Nuclear shape has fundamental functional implications, as in immune cell infiltration, cancer metastasis and stem cell differentiation. We are studying how the nuclear envelope deformation process is regulated at molecular level, especially by high-throughput experimental screening methods and data analysis.
- Martewicz S, Luni C, Zhu X, Cui M, Hu M, Qu S, Buratto D, Yang G, Grespan E, Elvassore N. 2020. "Nuclear morphological remodeling in human granulocytes is linked to prenylation independently from cytoskeleton". Cells-Basel 9:2509.
- Martewicz S, Zhu X, Qu S, Cui M, Grespan E, Luni C, Jiang B, Yang G, Elvassore N. 2022. "Micropillar based phenotypic screening platform uncovers involvement of HDAC2 in nuclear deformability". Biomaterials 286:121564.
Self-regulation along human somatic cell reprogramming to pluripotency. In view of personalized medicine, human pluripotent stem cells, obtained by somatic cell reprogramming, play a major role. We are interested in understanding how extra-cellular endogenous regulation affects the reprogramming process and strongly enhances its efficiency.
- Luni C*, Giulitti S*, Serena E, Ferrari L, Zambon A, Gagliano O, Giobbe GG, Michielin F, Knöbel S, Bosio A, Elvassore N. 2016. "High-efficiency cellular reprogramming with microfluidics". Nature Methods 13(5):446-452.
- Gagliano O*, Luni C*, Qin W, Bertin E, Torchio E, Galvanin S, Urciuolo A, Elvassore N. 2019. "Microfluidic reprogramming to pluripotency of human somatic cells". Nature Protocols 14:722–737.
- Luni C, Gagliano O, Elvassore N. 2022. "Derivation and differentiation of human pluripotent stem cells in microfluidic devices". Annu Rev Biomed Eng 24:231-248.
- Cesare E*, Urciuolo A*, Stuart HT*, Torchio E, Gesualdo A, Laterza C, Gagliano O, Martewicz S, Cui M, Manfredi A, Di Filippo L, Sabatelli P, Squarzoni S, Zorzan I, Betto RM, Martello G, Cacchiarelli D, Luni C, Elvassore N. 2022. "3D ECM-rich environment sustains the identity of naive human iPSCs". Cell Stem Cell 29(12):1703-1717.e7.
- Panariello F*, Gagliano O*, Luni C*, Grimaldi A*, Angiolillo S, Qin Wei, Manfredi A, Annunziata P, Slovin S, Vaccaro L, Riccardo S, Bouche V, Dionisi M, Salvi M, Martewicz S, Hu M, Cui M, Stuart H, Laterza C, Baruzzo G, Schiebinger G, Di Camillo B, Cacchiarelli D, Elvassore N. 2023 "Cellular population dynamics shape the route to human pluripotency". Nature Communications 14:2829-2843.
