- Docente: Tatiana Gianni
- Credits: 6
- SSD: BIO/19
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Biological Sciences (cod. 8012)
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from Sep 18, 2024 to Dec 19, 2024
Learning outcomes
The main educational objective of the course is the study of the interactions of microorganisms with the surrounding environment, with an in-depth study of microbiological and molecular techniques used in general microbiology and microbial ecology. Specific objectives concern the acquisition of theoretical knowledge (frontal modules) and practical (laboratory modules) for the characterization of microbial ecosystems, for the analysis of biogeochemical cycles and the study of microbial development in biofilms.
Course contents
Methods of analysis of microbial communities:
- Study of culture-dependent methods of various microorganisms that are distinguished by the request for particular growth conditions
- microscopic techniques used to identify both the various organisms and their components (pili, capsule ...)
-Analysis of the biochemical assays used to discriminate the various microorganisms
-Molecular assays
Interactions between microorganisms and plants (phyllosphere, rhizosphere, - mycorrhizae, - nitrogen fixation, - plant pathogens) and use of microorganisms in biological control (biological control).
Use of microorganisms in a number of applications: bioremediation, vaccine production etc.
Cell colture
Virus culture
Laboratory program
Bacterial transformation and inducible proteins
Tests aimed at identifying the various microorganisms: selective and differential media, oxidase tests; catalase test, fermentation test etc...
C-ELISA TEST
Enzyme immunostaining for identification of viral infection
Readings/Bibliography
The slides and notes taken in class are essential for the preparation of the exam.
As a reference, the student can deepen the topics on the following text:
Brock: Biology of Microorganisms (general, environmental and industrial microbiology) MT. Madigan, JM. Martinko, DS. Stahl, KS. Bender, DH. Buckley 14 / Ed. Pearson.
Teaching methods
Lectures on the topics of the course on the basis of online material, possible presentation to groups of papers of interest.
Laboratory experiences in pairs.
Assessment methods
Written exam with some open-ended questions and a series of multiple choice questions.
Any oral questions if further verification is required.
Teaching tools
The teaching material presented in class will be made available to the student in electronic format.
Office hours
See the website of Tatiana Gianni