07201 - Microbiology and Veterinary Immunology

Learning outcomes

At the completion of the course the student should be able to understand the basic principles of microbiology with an appreciation of the variety of bacteria and viruses, their modes of existence and how they relate to health and disease of animals. The student should be able to describe the methods used in the diagnosis of infectious diseases and to describe the functions of the immune system and explain the principles of immunization.

Course contents

Microorganisms and diseases: Koch's postulates. Study of microbial structure: microscopy . Procaryotic cell structure. Microbial nutrition and growth : common nutrient requirements, culture media, isolation of pure cultures, the growth curve, the influence of environmental factors on growth, measurement of microbial growth. Control of microorganisms by physical and chemical agents. The pattern of microbial death. Microorganism and host: basic concept of infectious disease, mechanisms of bacterial virulence, bacterial toxins. Taxonomy and classification of bacteria, phenotypic and genotypic methods. Bacterial genetics: mutations and recombination. Antibiotic resistance, minimum inhibitory concentration, disc diffusion method. Bacterial taxa of veterinary concern.General properties of viruses. Structure of viruses. Cultivation of viruses, methods of titration. Principles of virus taxonomy. Classification of animal viruses. Replication of animal viruses. Virus genetics. Viral taxa of veterinary concern. Introduction to immunology. General properties of immune responses. Antibodies: structure and functions. Antigens. Antibody binding of antigens. Antigen receptors and accessory molecules of T lymphocytes. The major histocompatibility complex. Antigen processing and presentation to T lymphocytes. Activation of B and T cells. Immunologic tolerance. Effector mechanisms of humoral and cell-mediated immunity. Serological tests. Immunity at body surfaces. Passive immunity: natural and artificial. Vaccines. Adjuvants. Classical and molecular methods in clinical microbiology.

Readings/Bibliography

Poli G., Cocilovo A., Dall’Ara P., Martino P.A., Ponti W.,  Microbiologia e immunologia veterinaria, II Edizione, UTET, Torino, 2005.

 Tizard I., Veterinary immunology: an introduction, VII Ed. Saunders Company, Philadelphia, 2004.

Teaching methods

The theoretical lectures take place in a teaching room properly supplied with multimedia equipment. The practical lectures, carried out in a properly equipped teaching lab, provide for a constant presence of the teacher and its co-operators together with the students, with introductive and explanatory discussions. Every practical training activity is carried out singly by the students and submitted to discussion with the teacher.

 

Assessment methods

The examination is oral and consists in several questions aimed at verifying the knowledge and the aptitude to link the different topics dealt with during the course.

 

 

Teaching tools

Light board, slide projector, PC video-projector.

Office hours

See the website of Renato Giulio Zanoni