- Docente: Giuseppe Gargiulo
- Credits: 5
- SSD: BIO/18
- Language: Italian
- Teaching Mode: In-person learning (entirely or partially)
- Campus: Bologna
- Corso: First cycle degree programme (L) in Biological sciences (cod. 0091)
Learning outcomes
The course will focus on the new genetic technologies as tools to further our understanding of genetic mechanisms and gene expression in eukaryotes.
Course contents
Recombinant DNA tecnology. Functional organization of the eukaryotic genome. Methodologies for genome analysis. Reverse genetics. Insertional mutagenesis and knock out mutants. Gene transfer in eukaryotic cells and transgenic organisms. Genetic control of animal development. Drosophila embryonic pattern formation. Formation of morphogenic gradients. mRNA localization and translational control. Establishment of the dorso-ventral and antero-posterior axes of symmetry. Homeotic and segmentation genes.
Readings/Bibliography
Il Gene , Benjamin Lewin, Zanichelli- Bologna;
Biologia della sviluppo, Gilbert Scott.F. Zanichelli- Bologna;
Biologia dello Sviluppo , Lewis Wolpert, Zanichelli
-Bologna;
DNA Ricombinante, Watson J.D. et al. Zanichelli-Bologna;
Some topics will require the study of the original papers.
Teaching methods
Lectures. Practical work in the lab (one credit ).
Assessment methods
Oral interview.
Teaching tools
power-point
Office hours
See the website of Giuseppe Gargiulo