- Docente: Donatella Serafini Fracassini
- Credits: 3
- SSD: BIO/01
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Biological sciences (cod. 0091)
Learning outcomes
The objective is the knowledge of detailed aspects of the
developmental biology either by oral presentations or experiments
performed in the laboratory. Students will personally acquire
experience of the techniques of in vitro cultures, which can be
utilised for the micro propagation industry, and the production of
transgenic plants.
Course contents
CYTOLOGY genetic stability. Somaclonal variation. Secondary
metabolites and applications. Cytoskeleton and its dynamic during
cell growth. Motility proteins. Architecture and biogenesis of the
cell wall. Origin, evolution and inter-conversion of chloroplasts.
Molecular aspects of programmed cell death. The transgenic plants:
theory and practical experiments.
THE IN VITRO COLTURES. The zygotic and somatic embryogenesis.
Meristems and staminal cells. The in vitro morphogenesis:
caulogenesis, rizogenesis and genesis of flowers. The model
systems.
METHODOLOGIES. preparation of the culture media, sterility
condition, preparation of explants of higher plants and algae.
Quantitative evaluation of growth and statistical analysis of the
data. Presentation of the data.
Readings/Bibliography
Altamura MM et al. Elementi di biologia dello sviluppo delle
piante. Editore EdiSES, 2007.
WOLPERT L. e altri - Biologia dello sviluppo, Editore Zanichelli,
2000.
BUCHANAN BB. e altri - Biochimica e Biologia Molecolare delle
Piante, Editore Zanichelli, 2003.
additional read: SALA F. - Gli OGM sono davvero pericolosi? Editori
Laterza, 2005.
Teaching methods
Oral presentations. Individual and group experimental work.
Assessment methods
Preparation of a power point on a subject of the development
biology. Presentation of the data obtained by the experiments
performed in the laboratory.
Teaching tools
Video projector for power points prepared by the teacher.
Autoclave, flow hood, thermostatic chambers, and microscope,
computer, PCR. Electrophoresis.
Office hours
See the website of Donatella Serafini Fracassini