- Docente: Patrizia Romualdi
- Credits: 4
- SSD: BIO/14
- Language: Italian
- Moduli: Patrizia Romualdi (Modulo 1) Monia Lenzi (Modulo 2)
- Teaching Mode: Traditional lectures (Modulo 1) Traditional lectures (Modulo 2)
- Campus: Bologna
- Corso: Single cycle degree programme (LMCU) in Pharmaceutical Chemistry and Technology (cod. 5986)
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from Oct 14, 2024 to Oct 17, 2024
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from Oct 28, 2024 to Oct 31, 2024
Learning outcomes
At the end of the course the student acquires the main scientific knowledge relating to the study of drugs both at a pharmacological and toxicological level; - knows the pharmacological and toxicological methods for the evaluation of drugs at a preclinical experimental level - acquires knowledge on the applicability of the disciplines in question, moving from theory to practice, then touching the main strategies for studying the pharmacological and toxic effects of molecules; - after a theoretical part, the student has the opportunity to carry out some practical experiences in a biological laboratory with the use of specific pharmacological and toxicological protocols
Course contents
Course contents
Module 1 Applied Pharmacology Prof. Patrizia Romualdi (2 CFU)
Frontal Lessons (1 CFU) : Use of in vitro and in vivo models for the study of drugs. Molecular biology methods applied to pharmacology; 3R-Rules for animal use; cell cultures, methods for the identification of proteins; Quantitation of nucleic acid methods, Drug effects on biological parameters; experimental protocols preparation; organization of a laboratory of experimental pharmacology for basic
Laboratory Training (1CFU) – Use of a cell culture in adhesion: maintenance and use. Protein extraction from cultured cells and quantification by BCA assay. Extraction of nucleic acid from cells, quantitative and qualitative analysis of nucleic acid by agarose gel electrophoresis and spectrophotometry. Retrotranscription from RNA into cDNA. Assessment of mRNA levels in neuronal cells of genes of interest by Real time-PCR. Analysis of experimental results.
Module 2 Applied Toxicology Prof. Fabiana Morroni (2 CFU)
Frontal Lessons (1 CFU): The Good Laboratory Practices (GLP) in testing chemicals. Animal testing. Alternative methods to animal testing. Organization of a Toxicology laboratory. Test systems.
Cytotoxicity. Short-term toxicology. Long-term toxicology. Genetic toxicology. Reproduction toxicity studies.
Laboratory Training (1CFU) - Cell culture: maintenance and use. Cell count and analysis of cell viability. Treatment with a test substance. Assessment of cell proliferation. Evaluation of the cytotoxicity of a xenobiotic. Learning to keep a good laboratory notebook.
Readings/Bibliography
Readings/Bibliography
Material provided by the Teachers
OECD guidelines
Teaching methods
Teaching methods
The course is planned in 8 hours of frontal lessons and 15 hours of experimental laboratory training for each module.
Assessment methods
Assessment methods
The exam will consist in an oral test aimed to assess the knowledge and comprehension of the students about the topics investigated during the course, and discussion on practical experiences carried out during the laboratory training. The Teachers will participate together to the Commitee.
Teaching tools
Teaching tools
PPT provided from the Teacher for the oral lessons and experimental protocols for the laboratory training.
Office hours
See the website of Patrizia Romualdi
See the website of Monia Lenzi
SDGs


This teaching activity contributes to the achievement of the Sustainable Development Goals of the UN 2030 Agenda.