39291 - Clinical Pathology Techniques (BO)

Learning outcomes

At the end of the course the student will acquire the principles used for the critical evaluation and interpretation of some clinical laboratory methods. 

Course contents

A brief history of the cardiovascular system; short survey on the microanatomy of the cardiovascular system: arteries, veins and capillaries. Vascular-wall (intima, media and adventitia): structure, function and cells. Vascular-wall stem cells. Isolation protocols. Mechanical and enzymatic methods. Cell culture. Characteristics of cell growth in culture. Culture media. Instrumentation. 3D  cultures. Cluster of Differentation. Main vascular cell immunophenotypes.  Methods for the morphological and phenotipic  characterization. Methods for detecting surface antigens. Cell riprogramming. iPSCs.

 

   

Readings/Bibliography

• G Pasquinelli - S Valente. Sistema vascolare e cellule staminali del sistema vascolare. In: Cellule Staminali. GP Bagnara (ed.) II ed. Ed. Esculapio, Bologna, 2016. pp 51-77.
  
  
• Guida all'uso delle Colture Cellulari. C. Ciavarella, G. Pasquinelli. 2019.

Teaching methods

The course consists of 2 CFU: frontal teaching, video on ptactical laboratory activities.

Assessment methods

The final exam consists of the oral presentation of two questions on the program's topics.
For each question, the student receives a vote based on the demonstrated ability to briefly expose the issues with some critical thinking. The weighted result, expressed in 30/30, contributes in proportion to the number of credits, the final vote of the entire integrated course.

Teaching tools

The teaching material presented in class will be made available in virtuale.unibo.it

Office hours

See the website of Sabrina Valente