66619 - Molecular Genetics

Learning outcomes

Students will acquire notions about the molecular genetics of model systems such as M. musculus and H. sapiens and the molecular methods to analyze DNA and RNA and generate mutants through gene inactivation and transgenesis.

Course contents

The course provides knowledge of modern molecular genetics approaches to analyze gene function in vivo, using model organisms. These approaches allow exploring the function of a specific gene in different tissues and cell types using mutations or inducing genetic manipulation. Furthermore, the use of molecular genetics techniques in the study of gene interactions with the aim to understand molecular mechanisms underlying biological processes will also be presented.

Readings/Bibliography

T. Strachan, Andrew P. Read "Human Molecular Genetics" V edition, Garland Science

Philip Meneely "Advanced Genetic Analysis", Oxford Press

Lectures slides and educational supports will be available for students attending the course on the Virtual Learning Environment (https://virtuale.unibo.it ).

Teaching methods

The course consists of 5 CFU of lectures and 1 CFU of lab activities. During the lectures the contents of the program will be illustrated using PowerPoint slides. The laboratory activities will focus on the use of methods to study gene expression.

Wetlab course NOTE! The wetlab training activity requires that students take modules 1 and 2 in e-learning mode [https://www.unibo.it/it/servizi-e-opportunita/salute-e-assistenza/salute-e-sicurezza/sicurezza-e-salute-nei-luoghi-di-studio-e-tirocinio] and participate to module 3 of specific training on safety and health in the workplace. Information on dates and attendance of module 3 can be found in the appropriate section of the course website.

Assessment methods

Oral exam to evaluate 1) the student’s knowledge of the contents developed during the course and 2) the student’s ability to interconnect the different topics.

Teaching tools

PowerPoint presentations, teaching materials. Laboratory activity.

Office hours

See the website of Valeria Cavaliere