- Docente: Stefano Masiero
- Credits: 3
- SSD: CHIM/06
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Single cycle degree programme (LMCU) in Pharmaceutical Chemistry and Technology (cod. 5986)
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from Mar 05, 2025 to Apr 15, 2025
Learning outcomes
The goal of the course is to provide the industrial pharmacist with the concepts and experimental tools used to investigate the supramolecular phenomena involved in drug/molecular target interactions. The student will familiarize with nucleic acids and proteins chemistry, the chemical and physical factors ruling supramolecular processes, the techniques used in supramolecular investigation.
Course contents
Supramolecular chemistry concepts: molecular recognition, cooperativity, self-assembly and self-organization.
Determination of binding constants: UV-Vis and NMR methods.
Calorimetric titrations (ITC).
NOE and diffusion NMR (DOSY) and their applications in supramolecular chemistry.
Circular dichroism for the investigation of biomolecules in solution and their supramolecular behaviour.
Aminoacids, peptides, proteins, nucleotides and oligonucleotides: preparation and supramolecular behaviour.
Nucleic acids and proteins sequencing.
Peptides and oligonucleotides as drugs: selected examples.
Readings/Bibliography
- J. W. Steed, J. L. Atwood - Supramolecular Chemistry-Wiley (2022)
- C.A. Schalley (ed.) Analytical Methods in Supramolecular Chemistry II ed. (2012) WILEY-VCH (D)
- T. D. W. Claridge - High-resolution NMR techniques in organic chemistry- II ed. (2009) Elsevier
- Blackburn G.M. e Gait, M.J. (eds.) Nucleic Acids in Chemistry and Biology II ed., (1996) o subsequent, Oxford University Press (UK).
- Gait, M.J. (ed.) Oligonucleotide Synthesis a Practical Approach (1984) IRL Press Oxford (UK).
- J. Jones, Aminoacid and peptide synthesis, Oxford University Press, 1992.
- M. Bodanszki, Principles of peptide synthesis, 2nd ed. Springer, 1993.
Teaching methods
Lectures
Assessment methods
The final examination, aiming at ascertaining the student's acquired skills, consists of an oral presentation in front of classmates, pertaining a specific topic related to the course main contents. The subject will be agreed between student and teacher, who will provide the relevant literature. The presentation will be followed by a brief discussion.
Teaching tools
Presentations. Files will be made available for download.
Office hours
See the website of Stefano Masiero