39315 - Cyto-histopathology Techniques (CE)

Learning outcomes

At the end of the course, the student acquires a basic preparation to set up and technically evaluate the result of cytological and histological preparations, to understand the motivations that guide the application of cytohistological techniques in diagnostics and to carry out the technical activity in the sector room.

Course contents

Intraoperative examination, sentinel lymph node of the breast performed by cryostat on frozen sections.

Sentinel lymph node protocols for breast and melanoma on paraffin sections.

Molecular morphology methods Enzymatic immunohistochemistry, immunohistochemical staining procedures:

• Preparation of biological samples
• Antibodies
• Antigenic unmasking
• Signal Detection Systems
• Protocol design: criticality, validation, quality controL

DNA amplification techniques by PCR: Real Time PCR methods,direct sequencing techniques and DNA pyrosequencing

Pre-Analytics & Sample Management

• Notes on the main techniques
• Key predictive markers
• Technological Innovations in Histopathology: Digital Pathology

Diagnostic cytology:

Applications of Diagnostic Cytology and Types of Sampling
Preparation of the various cytological samples
Fixation
Colors
Immunocytochemistry on stained specimens and preparations
HPV-DNA by real-time PCR in the management of cervico-vaginal screening

The Sector Room

Readings/Bibliography

Ruco L., Scarpa A. Anatomia Patologica Le basi I Edizione (2007) ristampa (2016) Edra

Suvarna K, Layton C, Bancroft J. Bancroft’s Theory and Practice of Histological Techniques – 8th edition (2019). Elsevier

Teaching methods

Lectures

Assessment methods

Oral exam

Teaching tools

Teaching material provided by the teacher (slides, guidelines, regulations).

Office hours

See the website of Ilaria Sampaoli