- Docente: Roberto Bernardoni
- Credits: 3
- SSD: BIO/18
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: First cycle degree programme (L) in Biological sciences (cod. 0091)
Learning outcomes
The purpose of this course is to introduce students to methods and applications of genetic engineering.
Course contents
Techniques used to obtain desired DNA fragments.
Site-directed mutagenesis.
Cloning vectors: plasmids, vectors based on the Lambda and P1 bacteriophages, cosmids. Artificial chromosomes: BACs and YACs.
Gene transfer methodologies in model organisms. P-element mediated germ line transformation in Drosophila; embryonic stem cell technology in mouse.
Transgenics and reverse genetic. Approaches to gene regulation and gene function.
Controlling transgene expression: inducible promoters, tissue-specific promoters, binary sistems of activation.
Gene silencing. Gene targenting. RNA interference.
Readings/Bibliography
GENETIC ENGINEERING: principles and techniques. Primrose S., Twyman R., Old B. ed. Zanichelli (Bologna)
Teaching methods
Lectures
Assessment methods
Written examination
Teaching tools
Power Point presentations.
Links to further information
http://www.dipartimentobiologia.it/research/rudellavalle.asp
Office hours
See the website of Roberto Bernardoni